Wo2015179452a1 fourier ptychographic microscopy with. Widefield, highresolution fourier ptychographic microscopy. Its defining characteristic is translational invariance, which means the interference patterns are generated by one constant function e. Structured illumination fluorescence fourier ptychographic. Widefield fourier ptychographic microscopy using laser. A gigapixel superscope for biomedicine guoan zheng, xiaoze ou, roarke horstmeyer, jaebum chung and changhuei yang a simple modification, combined with computational optics, can boost the resolving power of standard microscopes by more than an order of magnitudeand open up new applications in both the lab and. So far, many supervised datadriven models have been applied to solve inverse imaging problems. Deep learning approach for fourier ptychography microscopy. The unique feature of the fpm is its capability to.
Resolutionenhanced fourier ptychographic microscopy. Highspeed fourier ptychographic microscopy based on. We report a computational 3d microscopy technique, termed fourier ptychographic diffraction tomography fpdt, that iteratively stitches together numerous variably illuminated, lowresolution images acquired with a lownumerical aperture na objective in 3d fourier space to create a wide fieldofview fov, highresolution, depth. Wide field, highresolution lensless onchip microscopy via nearfield blind ptychographic modulation shaowei jiang, a jiakai zhu, a pengming song, b chengfei guo, a zichao bian, a ruihai wang, a yikun huang, a shiyao wang, c he zhang a and guoan zheng ab. Fourier ptychographic microscopy fpm is a recently developed technique for highresolution complex imaging with a wide fieldofview. Pengming song, shaowei jiang, he zhang, zichao bian. Fourier ptychographic microscopy fpm is a recently developed technique stitching lowresolution images in fourier domain to realize wide field highresolution imaging. In this article, we report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated, lowresolution intensity images in fourier space to produce a wide field, highresolution complex sample image. Fpm combines captured lowresolution images corresponding to anglevarying illuminations in the fourier domain with phase retrieval and synthetic aperture algorithms. Fourier ptychographic fp microscopy is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple lowbandwidth images captured at different spatial frequency regions. A system and methods for wide field of view, high resolution fourier ptychographic microscopic imaging with a programmable led array light source. Coherent modulation imaging cmi has been shown to be an effective lensless diffraction approach to imaging general extended samples with fast algori. In this article, we report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated. Adaptive and efficient fourier ptychographic microscopy.
Guoan zheng, roarke horstmeyer, changhuei yang download pdf. A simply equipped fourier ptychography platform based on. The aim of this work is to develop a novel cnn framework to reconstruct video sequences of dynamic live cells captured using a computational microscopy technique, fourier ptychographic microscopy fpm. We report a computational 3d microscopy technique, termed fourier ptychographic diffraction tomography fpdt, that iteratively stitches together numerous variably illuminated, lowresolution images acquired with a lownumerical aperture na objective in 3d fourier space to create a wide fieldofview fov, highresolution, depthresolved complex refractive index ri. Fourier ptychographic microscopy, structured illumination microscopy, high resolution, wide field abstract. In this article, we report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated, lowresolution intensity images in fourier space to produce a wide field, high. Request pdf on may 1, 2020, chao zuo and others published widefield high resolution 3d microscopy with fourier ptychographic diffraction tomography. Gigapixel, chipscale and multifunctional microscopy. In this paper, a novel microscopic imaging technology, termed light field fourier ptychographic microscopy lffpm, is presented which enables the reconstruction of wide field, highresolution images with the depth information by utilizing a lightemittingdiode led illumination matrix.
In this paper, we propose a novel framework which combines fourier ptychographic microscopy fpm and soyou only look once yolo for wcb detection. Guoan zheng, seung ah lee, yaron antebi, micheal b. Elowitz, and changhuei yang, the epetri dish, an onchip cellimaging platform based on subpixel perspective sweeping microscopy. Among different approaches, fourier ptychographic microscopy fpm is probably the most promising technique to realize highthroughput qpi by synthesizing a wide field, highresolution complex. Optimized denoising method for fourier ptychographic.
Us20170146788a1 fourier ptychographic microscopy with. Pdf we present an advanced lowcost widefield, highresolution computational microscope kit based on fourier ptychography. Dexin ye, guoan zheng, jingyu wang, zhiyu wang, shan qiao, jiangtao huangfu, and lixin ran, negative group velocity in the absence of absorption resonance, scientific. Aperturescanning fourier ptychography for 3d refocusing and superresolution macroscopic imaging siyuan dong,1,5 roarke horstmeyer,3,5 radhika shiradkar,1,5 kaikai guo,1 xiaoze ou,3 zichao bian,1 huolin xin,4 and guoan zheng1,2, 1biomedical engineering, university of connecticut, storrs, connecticut 06269, usa 2electrical and computer engineering, university. This compromise is conventionally defeated by using a high na objective with a small fov and then mechanically scan the sample in order to acquire separate images. Fourier plane imaging microscopy journal of applied physics 116, 103102 2014. The individual lights in the led array can be actuated according to random, nonrandom and hybrid random and nonrandom illumination strategies to produce high resolution images with fast acquisition speeds. Wide fieldofview reflectionmode ptychographic imaging. Fourier ptychography microscopy fpm is a recently emerged. Video below shows the operation of the fourier ptychographic microscope. Widefield, highresolution lensless onchip microscopy via near field blind. By adopting a wavefront correction strategy, the fpm method can also correct for aberrations and. Widefield highresolution 3d microscopy with fourier. Wide field highresolution 3d microscopy with fourier ptychographic diffraction tomography article pdf available in optics and lasers in engineering 1285.
Its principle is to realize the aperture expansion by using a phase retrieval algorithm to splice a set of lowresolution images under different illumination angles in the fourier domain. Google scholar profile book guoan zheng, fourier ptychographic imaging. Fourier ptychographic microscopy fpm1, based on multiangle illumination and fourier ptychographic algorithm, is a novel imaging method to achieve wide field and highresolution effect. Guoan zheng, roarke horstmeyer, and changhuei yang electrical engineering, california institute of technology, pasadena, ca 91125, usa. Conventional light field microscope lacks the ability of high lateral. It generates images by processing many coherent interference patterns that have been scattered from an object of interest. Guoan zheng, roarke horstmeyer and changhuei yang, wide field, highresolution fourier ptychographic microscopy, nature photonics, 7, 739745 20 featured by nature pdf. However, the timeconsuming process of image acquisition greatly narrows its applications in dynamic imaging. A wide field, highresolution imaging scheme that offers enhanced depth of field is demonstrated. Guoan zheng, roarke horstmeyer and changhuei yang, wide field, highresolution fourier ptychographic microscopy, nature photonics, 7, 739745 20.
The corresponding captured lowresolution images will be used to recover the highresolution complex object image. Widefield, highresolution lensless onchip microscopy. Fourier ptychographic microscopy fpm 1 is a widefield imaging technique in which a high resolution complex image is reconstructed by combining information from low resolution brightfield and darkfield images captured under different illumination angles fig. Pdf lowcost fourier ptychography microscope kit researchgate. A correction to the article wide field, highresolution fourier ptychographic microscopy that was published in the 2015 issue is presented which discusses the halfpitch resolution of microscope. The approach relies on stitching together timemultiplexed images in fourier. Guoan zheng, roarke horstmeyer, and changhuei yang. Highquality reconstruction of coherent modulation imaging. Medical research and clinical diagnostics require imaging of large sample areas with subcellular resolution. Conventional imaging techniques can provide either highresolution or wide fieldofview fov but not both.
Fourier ptychography fp is a newly developed computational imaging approach that achieves both high resolution and wide field of view by stitching a series of lowresolution images captured under anglevaried illumination. Soyolo based wbc detection with fourier ptychographic. Waller, multiplexed coded illumination for fourier ptychography with an led array microscope, biomedical optics express, vol. Widefield highresolution 3d microscopy with fourier ptychographic.
Fpm is a recently developed microscope technology which can achieve highresolution, wide field, and quantitative phase imaging at the same time without mechanical moving and phase. Fourier ptychographic microscopy fpm is a highresolution computational imaging technique that is robust to recover highresolution image and large sbp by anglevaried illumination 4,5,6. Wide field, highresolution fourier ptychographic microscopy fpm guoan zheng1, 2, xiaoze ou1, roarke horstmeyer1 and changhuei yang1 1electrical engineering, california institute of technology, pasadena, ca, 91125, usa 2biomedical engineering and electrical engineering, university of connecticut, storrs, connecticut, 06269, usa wide fieldofview. Aperturescanning fourier ptychography for 3d refocusing. Reflective mode fourier ptychography by basel salahieh. Mafpm configuration utilises an array of objective lenses coupled with detectors to. Fourier ptychographic microscopy using wavelength multiplexing. We report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated, lowresolution intensity images in fourier space to produce a wide field, highresolution complex sample image. Each time, we will turn on one led element and illuminate the sample from one incident angle. Wide field, highresolution fourier ptychographic microscopy. Wide fieldofview reflectionmode ptychographic imaging microscope with tabletop 12. Convolutional neural networks cnns have gained tremendous success in solving complex inverse problems. Guoan zheng, roarke horstmeyer and changhuei yang, widefield, high resolution fourier ptychographic microscopy.
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